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Isolation of Sperm Cells from the Pollen of Plumbago zeylanica
Intact sperm cells of Plumbago zeylanica were released from mature pollen grains near anthesis using osmotic shock with 20% sucrose. Sperm cell yields of up to 75% can be attained by differential centrifugation using a clinical centrifuge with concentrations of up to 8.8 × 10(6) cells/milliliter. Su...
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| Autor principal: | |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
1986
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1075328/ https://ncbi.nlm.nih.gov/pubmed/16664799 |
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