Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coli Cells

The relationship between the detection of mRNA and cellular viability in Escherichia coli was investigated in cells killed by heat or ethanol. Reverse transcription-PCR (RT-PCR) methods were developed for detecting mRNA from rpoH, groEL, and tufA genes. mRNA from all three genes was detected immedia...

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Bibliografske podrobnosti

izdano v:	Appl Environ Microbiol
Principais autores:	Sheridan, G. E. C., Masters, C. I., Shallcross, J. A., Mackey, B. M.
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1998
Teme:	Genetics and Molecular Biology
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106147/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9546166/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.4.1313-1318.1998
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