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Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coli Cells

The relationship between the detection of mRNA and cellular viability in Escherichia coli was investigated in cells killed by heat or ethanol. Reverse transcription-PCR (RT-PCR) methods were developed for detecting mRNA from rpoH, groEL, and tufA genes. mRNA from all three genes was detected immedia...

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Bibliografische gegevens
Hoofdauteurs: Sheridan, G. E. C., Masters, C. I., Shallcross, J. A., Mackey, B. M.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 1998
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC106147/
https://ncbi.nlm.nih.gov/pubmed/9546166
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