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Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coli Cells

The relationship between the detection of mRNA and cellular viability in Escherichia coli was investigated in cells killed by heat or ethanol. Reverse transcription-PCR (RT-PCR) methods were developed for detecting mRNA from rpoH, groEL, and tufA genes. mRNA from all three genes was detected immedia...

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Detaylı Bibliyografya
Yayımlandı:Appl Environ Microbiol
Asıl Yazarlar: Sheridan, G. E. C., Masters, C. I., Shallcross, J. A., Mackey, B. M.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology (ASM) 1998
Konular:
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106147/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9546166/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.4.1313-1318.1998
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