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Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coli Cells
The relationship between the detection of mRNA and cellular viability in Escherichia coli was investigated in cells killed by heat or ethanol. Reverse transcription-PCR (RT-PCR) methods were developed for detecting mRNA from rpoH, groEL, and tufA genes. mRNA from all three genes was detected immedia...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology
1998
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC106147/ https://ncbi.nlm.nih.gov/pubmed/9546166 |
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