Reverse Transcriptase (RT) Inhibition of PCR at Low Concentrations of Template and Its Implications for Quantitative RT-PCR

Numerous instances of reverse transcriptase (RT) inhibition of the PCR were observed while developing nonquantitative uncoupled RT-PCR techniques for detecting nitrogenase and ammonia monooxygenase gene expression in situ. The inhibitory effect of RT on the PCR was removed with increasing template c...

Descrizione completa

Dettagli Bibliografici

Pubblicato in:	Appl Environ Microbiol
Autori principali:	Chandler, Darrell P., Wagnon, Christina A., Bolton, Harvey
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1998
Soggetti:	Methods
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106100/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9464406/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.2.669-677.1998
Tags:	Aggiungi Tag Nessun Tag, puoi essere il primo ad aggiungerne!!