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Reverse Transcriptase (RT) Inhibition of PCR at Low Concentrations of Template and Its Implications for Quantitative RT-PCR

Numerous instances of reverse transcriptase (RT) inhibition of the PCR were observed while developing nonquantitative uncoupled RT-PCR techniques for detecting nitrogenase and ammonia monooxygenase gene expression in situ. The inhibitory effect of RT on the PCR was removed with increasing template c...

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Udgivet i:Appl Environ Microbiol
Principais autores: Chandler, Darrell P., Wagnon, Christina A., Bolton, Harvey
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1998
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Methods
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106100/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9464406/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.2.669-677.1998
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