Overexpression, Purification, and Characterization of the Cloned Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia

The metallo-β-lactamase L1 from Stenotrophomonas maltophilia was cloned, overexpressed, and characterized by spectrometric and biochemical techniques. Results of metal analyses were consistent with the cloned enzyme having 2 mol of tightly bound Zn(II) per monomer. Gel filtration chromatography demo...

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Detalhes bibliográficos

Publicado no:	Antimicrob Agents Chemother
Principais autores:	Crowder, Michael W., Walsh, Timothy R., Banovic, Linda, Pettit, Margaret, Spencer, James
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Mechanisms of Resistance
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105568/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9559809/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.42.4.921
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