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Overexpression, Purification, and Characterization of the Cloned Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia

The metallo-β-lactamase L1 from Stenotrophomonas maltophilia was cloned, overexpressed, and characterized by spectrometric and biochemical techniques. Results of metal analyses were consistent with the cloned enzyme having 2 mol of tightly bound Zn(II) per monomer. Gel filtration chromatography demo...

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Detalhes bibliográficos
Main Authors: Crowder, Michael W., Walsh, Timothy R., Banovic, Linda, Pettit, Margaret, Spencer, James
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC105568/
https://ncbi.nlm.nih.gov/pubmed/9559809
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