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Purification and Characterization of Gibberellic Acid-Induced Cysteine Endoproteases in Barley Aleurone Layers

Using in series ammonium sulfate precipitation, gel filtration, and DEAE anion exchange high performance liquid chromatography, we have purified to homogeneity a protease of M(r) 37,000 secreted from barley (Hordeum vulgare L. cv Himalaya) embryoless half-seeds. This protease exists in three isozymi...

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Detalhes bibliográficos
Main Authors: Koehler, Susan, Ho, Tuan-Hua David
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1054705/
https://ncbi.nlm.nih.gov/pubmed/16666134
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