Detection of Lassa Virus Antinucleoprotein Immunoglobulin G (IgG) and IgM Antibodies by a Simple Recombinant Immunoblot Assay for Field Use

The nucleoprotein of Lassa virus, strain Josiah, was expressed in Escherichia coli as an N-terminally truncated, histidine-tagged recombinant protein. Following affinity purification the protein was completely denatured and spotted onto nitrocellulose membrane. A total of 1 μg of protein was applied...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	ter Meulen, J., Koulemou, K., Wittekindt, T., Windisch, K., Strigl, S., Conde, S., Schmitz, H.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Virology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105290/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9774554/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.11.3143-3148.1998
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