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Detection of Lassa Virus Antinucleoprotein Immunoglobulin G (IgG) and IgM Antibodies by a Simple Recombinant Immunoblot Assay for Field Use

The nucleoprotein of Lassa virus, strain Josiah, was expressed in Escherichia coli as an N-terminally truncated, histidine-tagged recombinant protein. Following affinity purification the protein was completely denatured and spotted onto nitrocellulose membrane. A total of 1 μg of protein was applied...

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Bibliografische gegevens
Hoofdauteurs: ter Meulen, J., Koulemou, K., Wittekindt, T., Windisch, K., Strigl, S., Conde, S., Schmitz, H.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 1998
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC105290/
https://ncbi.nlm.nih.gov/pubmed/9774554
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