A PCR Specific for Escherichia coli O157 Based on the rfb Locus Encoding O157 Lipopolysaccharide

A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected <1 CFU of E. coli O157 per ml in raw...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Desmarchelier, Patricia M., Bilge, Sima S., Fegan, Narelle, Mills, Leanne, Vary, James C., Tarr, Phillip I.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1998
Sujets:	Bacteriology
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104928/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9620428/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.6.1801-1804.1998
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