A PCR Specific for Escherichia coli O157 Based on the rfb Locus Encoding O157 Lipopolysaccharide

A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected <1 CFU of E. coli O157 per ml in r...

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Autors principals: Desmarchelier, Patricia M., Bilge, Sima S., Fegan, Narelle, Mills, Leanne, Vary, James C., Tarr, Phillip I.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1998
Matèries:
Bacteriology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC104928/
https://ncbi.nlm.nih.gov/pubmed/9620428
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