Purification of P(II) and P(II)-UMP and In Vitro Studies of Regulation of Glutamine Synthetase in Rhodospirillum rubrum
The P(II) protein from Rhodospirillum rubrum was fused with a histidine tag, overexpressed in Escherichia coli, and purified by Ni(2+)-chelating chromatography. The uridylylated form of the P(II) protein could be generated in E. coli. The effects on the regulation of glutamine synthetase by P(II), P...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1999
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC103790/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10515945/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.20.6524-6529.1999 |
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