Purification of P(II) and P(II)-UMP and In Vitro Studies of Regulation of Glutamine Synthetase in Rhodospirillum rubrum
The P(II) protein from Rhodospirillum rubrum was fused with a histidine tag, overexpressed in Escherichia coli, and purified by Ni(2+)-chelating chromatography. The uridylylated form of the P(II) protein could be generated in E. coli. The effects on the regulation of glutamine synthetase by P(II), P...
Na minha lista:
| Udgivet i: | J Bacteriol |
|---|---|
| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1999
|
| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC103790/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10515945/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.20.6524-6529.1999 |
| Tags: |
Ingen Tags, Vær først til at tagge denne postø!
|