Direct Selection of IS903 Transposon Insertions by Use of a Broad-Host-Range Vector: Isolation of Catalase-Deficient Mutants of Actinobacillus actinomycetemcomitans

Transposon mutagenesis in bacteria generally requires efficient delivery of a transposon suicide vector to allow the selection of relatively infrequent transposition events. We have developed an IS903-based transposon mutagenesis system for diverse gram-negative bacteria that is not limited by trans...

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Pubblicato in:	J Bacteriol
Autori principali:	Thomson, Valeri J., Bhattacharjee, Mrinal K., Fine, Daniel H., Derbyshire, Keith M., Figurski, David H.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1999
Soggetti:	Genetics and Molecular Biology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC103693/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10572134/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.23.7298-7307.1999
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