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Direct Selection of IS903 Transposon Insertions by Use of a Broad-Host-Range Vector: Isolation of Catalase-Deficient Mutants of Actinobacillus actinomycetemcomitans

Transposon mutagenesis in bacteria generally requires efficient delivery of a transposon suicide vector to allow the selection of relatively infrequent transposition events. We have developed an IS903-based transposon mutagenesis system for diverse gram-negative bacteria that is not limited by trans...

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Detalhes bibliográficos
Main Authors: Thomson, Valeri J., Bhattacharjee, Mrinal K., Fine, Daniel H., Derbyshire, Keith M., Figurski, David H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1999
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC103693/
https://ncbi.nlm.nih.gov/pubmed/10572134
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