A new label technology for the detection of specific polymerase chain reaction products in a closed tube

A novel signal generation principle suitable for real time and end-point detection of specific PCR products in a closed tube is described. Linear DNA probes were labeled at their 5′-ends with a stable, fluorescent terbium chelate. The fluorescence intensity of this chelate is lower when it is couple...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Nurmi, Jussi, Ylikoski, Alice, Soukka, Tero, Karp, Matti, Lövgren, Timo
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2000
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102832/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10734205/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.8.e28
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