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A new label technology for the detection of specific polymerase chain reaction products in a closed tube

A novel signal generation principle suitable for real time and end-point detection of specific PCR products in a closed tube is described. Linear DNA probes were labeled at their 5′-ends with a stable, fluorescent terbium chelate. The fluorescence intensity of this chelate is lower when it is couple...

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Bibliographic Details
Main Authors: Nurmi, Jussi, Ylikoski, Alice, Soukka, Tero, Karp, Matti, Lövgren, Timo
Format: Artigo
Language:Inglês
Published: Oxford University Press 2000
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC102832/
https://ncbi.nlm.nih.gov/pubmed/10734205
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