New chromatographic and biochemical strategies for quick preparative isolation of tRNA

A combination of hydrophobic chromatography on phenyl-Sepharose and reversed phase HPLC was used to purify individual tRNAs with high specific activity. The efficiency of chromatographic separation was enhanced by biochemical manipulations of the tRNA molecule, such as aminoacylation, formylation of...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Cayama, Edmundo, Yépez, Antonio, Rotondo, Fernando, Bandeira, Eduardo, Ferreras, Ana C., Triana-Alonso, Francisco J.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2000
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102749/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871387/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.12.e64
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