New chromatographic and biochemical strategies for quick preparative isolation of tRNA

A combination of hydrophobic chromatography on phenyl-Sepharose and reversed phase HPLC was used to purify individual tRNAs with high specific activity. The efficiency of chromatographic separation was enhanced by biochemical manipulations of the tRNA molecule, such as aminoacylation, formylation of...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Cayama, Edmundo, Yépez, Antonio, Rotondo, Fernando, Bandeira, Eduardo, Ferreras, Ana C., Triana-Alonso, Francisco J.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2000
Témata:	NAR Methods Online
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102749/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871387/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.12.e64
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