Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer
In the present report we show that unknown DNA fragments are easily amplified in a single PCR reaction from an oligo-cassette library with a single genome-specific primer in combination with a cassette-specific primer. The novelty of the system, in comparison to the vectorette PCR method, lies in th...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2000
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102640/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871354/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.11.e55 |
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