Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer

In the present report we show that unknown DNA fragments are easily amplified in a single PCR reaction from an oligo-cassette library with a single genome-specific primer in combination with a cassette-specific primer. The novelty of the system, in comparison to the vectorette PCR method, lies in th...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Kilstrup, Mogens, Kristiansen, Klaus N.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2000
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102640/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871354/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.11.e55
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