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Identification of a base-specific contact between the restriction endonuclease SsoII and its recognition sequence by photocross-linking

A target sequence-specific DNA binding region of the restriction endonuclease SsoII was identified by photocross-linking with an oligodeoxynucleotide duplex which was substituted with 5-iododeoxyuridine (5-IdU) at the central position of the SsoII recognition site (CCNGG). For this purpose the SsoII...

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Detalhes bibliográficos
Main Authors: Kubareva, Elena A., Thole, Hubert, Karyagina, Anna S., Oretskaya, Tatiana S., Pingoud, Alfred, Pingoud, Vera
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2000
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC102617/
https://ncbi.nlm.nih.gov/pubmed/10666447
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