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Optimized synthesis of phosphorothioate oligodeoxyribonucleotides substituted with a 5′-protected thiol function and a 3′-amino group

A new deprotection procedure enables a medium scale preparation of phosphodiester and phosphorothioate oligonucleotides substituted with a protected thiol function at their 5′-ends and an amino group at their 3′-ends in good yield (up to 72 OD units/µmol for a 19mer phosphorothioate). Syntheses of 3...

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Detalhes bibliográficos
Main Authors: Aubert, Yves, Bourgerie, Sylvain, Meunier, Laurent, Mayer, Roger, Roche, Annie-Claude, Monsigny, Michel, Thuong, Nguyen T., Asseline, Ulysse
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC102547/
https://ncbi.nlm.nih.gov/pubmed/10637335
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