The efficiency of Escherichia coli selenocysteine insertion is influenced by the immediate downstream nucleotide
Selenocysteine (Sec) incorporation requires the TGA opal codon and a downstream Sec insertion sequence (SECIS), which can be partially randomized and cloned into M13 pIII fusion constructs for phage display. This combinatorial approach provides a convenient non-radioactive assay that couples phage p...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102542/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10637327/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.3.755 |
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