The efficiency of Escherichia coli selenocysteine insertion is influenced by the immediate downstream nucleotide

Selenocysteine (Sec) incorporation requires the TGA opal codon and a downstream Sec insertion sequence (SECIS), which can be partially randomized and cloned into M13 pIII fusion constructs for phage display. This combinatorial approach provides a convenient non-radioactive assay that couples phage p...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Sandman, Karen E., Noren, Christopher J.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2000
Soggetti:	Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102542/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10637327/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.3.755
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