Megaplasmid pRme2011a of Sinorhizobium meliloti Is Not Required for Viability

We report the curing of the 1,360-kb megaplasmid pRme2011a from Sinorhizobium meliloti strain Rm2011. With a positive selection strategy that utilized Tn5B12-S containing the sacB gene, we were able to cure this replicon by successive rounds of selecting for deletion formation in vivo. Subsequent So...

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Bibliografski detalji

Izdano u:	J Bacteriol
Glavni autori:	Oresnik, Ivan J., Liu, Shu-Lin, Yost, Christopher K., Hynes, Michael F.
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 2000
Teme:	Plant Microbiology
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC101967/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10852892/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.12.3582-3586.2000
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