Megaplasmid pRme2011a of Sinorhizobium meliloti Is Not Required for Viability

We report the curing of the 1,360-kb megaplasmid pRme2011a from Sinorhizobium meliloti strain Rm2011. With a positive selection strategy that utilized Tn5B12-S containing the sacB gene, we were able to cure this replicon by successive rounds of selecting for deletion formation in vivo. Subsequent So...

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Bibliografske podrobnosti
Main Authors: Oresnik, Ivan J., Liu, Shu-Lin, Yost, Christopher K., Hynes, Michael F.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2000
Teme:
Plant Microbiology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC101967/
https://ncbi.nlm.nih.gov/pubmed/10852892
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