Megaplasmid pRme2011a of Sinorhizobium meliloti Is Not Required for Viability
We report the curing of the 1,360-kb megaplasmid pRme2011a from Sinorhizobium meliloti strain Rm2011. With a positive selection strategy that utilized Tn5B12-S containing the sacB gene, we were able to cure this replicon by successive rounds of selecting for deletion formation in vivo. Subsequent So...
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| Pubblicato in: | J Bacteriol |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology (ASM)
2000
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC101967/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10852892/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.12.3582-3586.2000 |
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