Isolation and Characterization of Mini-Tn5Km2 Insertion Mutants of Enterohemorrhagic Escherichia coli O157:H7 Deficient in Adherence to Caco-2 Cells

Adherence of enterohemorrhagic Escherichia coli (EHEC) to intestinal epithelium is essential for initiation of the infection. To identify genes involved in adherence, an EHEC O157:H7 strain (O157Sakai) was mutagenized by mini-Tn5Km2, where Km refers to kanamycin resistance, and 4,677 insertion mutan...

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Detalhes bibliográficos
Main Authors: Tatsuno, Ichiro, Kimura, Hiroshi, Okutani, Akiko, Kanamaru, Kyoko, Abe, Hiroyuki, Nagai, Shinya, Makino, Kozo, Shinagawa, Hideo, Yoshida, Mitsutaka, Sato, Katsuhiro, Nakamoto, Jyunichi, Tobe, Toru, Sasakawa, Chihiro
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Molecular and Cellular Pathogenesis
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC101558/
https://ncbi.nlm.nih.gov/pubmed/10992506
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