Isolation and Characterization of Mini-Tn5Km2 Insertion Mutants of Enterohemorrhagic Escherichia coli O157:H7 Deficient in Adherence to Caco-2 Cells

Adherence of enterohemorrhagic Escherichia coli (EHEC) to intestinal epithelium is essential for initiation of the infection. To identify genes involved in adherence, an EHEC O157:H7 strain (O157Sakai) was mutagenized by mini-Tn5Km2, where Km refers to kanamycin resistance, and 4,677 insertion mutan...

Full description

Saved in:
Bibliographic Details
Main Authors: Tatsuno, Ichiro, Kimura, Hiroshi, Okutani, Akiko, Kanamaru, Kyoko, Abe, Hiroyuki, Nagai, Shinya, Makino, Kozo, Shinagawa, Hideo, Yoshida, Mitsutaka, Sato, Katsuhiro, Nakamoto, Jyunichi, Tobe, Toru, Sasakawa, Chihiro
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
Molecular and Cellular Pathogenesis
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC101558/
https://ncbi.nlm.nih.gov/pubmed/10992506
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items