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Mutational analysis defines the 5′-kinase and 3′-phosphatase active sites of T4 polynucleotide kinase
T4 polynucleotide kinase (Pnk) is a bifunctional 5′-kinase/3′-phosphatase that aids in the repair of broken termini in RNA by converting 3′-PO(4)/5′-OH ends into 3′-OH/5′-PO(4) ends, which are then sealed by RNA ligase. Here we have employed site-directed mutagenesis (introducing 31 mutations at 16...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2002
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC100346/ https://ncbi.nlm.nih.gov/pubmed/11842120 |
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