Mutational analysis defines the 5′-kinase and 3′-phosphatase active sites of T4 polynucleotide kinase
T4 polynucleotide kinase (Pnk) is a bifunctional 5′-kinase/3′-phosphatase that aids in the repair of broken termini in RNA by converting 3′-PO(4)/5′-OH ends into 3′-OH/5′-PO(4) ends, which are then sealed by RNA ligase. Here we have employed site-directed mutagenesis (introducing 31 mutations at 16...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2002
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC100346/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11842120/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.4.1073 |
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