Ordered catenation of sequence-tagged sites and multiplexed SNP genotyping by sequencing

We describe a method for the efficient genotyping of SNPs, involving sequencing of ordered and catenated sequence-tagged sites (OCS). In OCS, short genomic segments, each containing an SNP, are amplified by PCR using primers that carry specially designed extra nucleotides at their 5′-ends. Amplifica...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Higasa, Koichiro, Hayashi, Kenshi
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2002
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC100310/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11809899/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.3.e11
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