Ordered catenation of sequence-tagged sites and multiplexed SNP genotyping by sequencing
We describe a method for the efficient genotyping of SNPs, involving sequencing of ordered and catenated sequence-tagged sites (OCS). In OCS, short genomic segments, each containing an SNP, are amplified by PCR using primers that carry specially designed extra nucleotides at their 5′-ends. Amplifica...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2002
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC100310/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11809899/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.3.e11 |
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