An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines

Summary: The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide a protocol describing the synthesis and testing of the FusXTBE plasmids in cultured human cell lines. This tool is...

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Main Authors: Bibekananda Kar, Ankit Sabharwal, Santiago Restrepo-Castillo, Brandon W. Simone, Karl J. Clark, Stephen C. Ekker
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2022-06-01
Colecção:STAR Protocols
Assuntos:
Cell Biology
Genetics
Sequencing
Molecular Biology
CRISPR
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S266616672200168X
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