An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2

Summary: Elucidating the molecular interactions between virus and host is fundamental to understanding the mechanism of viral pathogenesis. Here, we present a protocol to screen SARS-CoV-2 protein interactors using an antibody-based TurboID proximity labeling approach. This technique directly identi...

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Bibliographic Details
Main Authors: Limin Shang, Yuehui Zhang, Yuchen Liu, Chaozhi Jin, Yanan Zhao, Jing Zhang, Pei-Hui Wang, Jian Wang
Format: Artigo
Language:Inglês
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Microbiology
Molecular/Chemical Probes
Protein Biochemistry
Proteomics
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002866
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