Single-Chain Lanthanide Luminescence Biosensors for Cell-Based Imaging and Screening of Protein-Protein Interactions

Summary: Lanthanide-based, Förster resonance energy transfer (LRET) biosensors enabled sensitive, time-gated luminescence (TGL) imaging or multiwell plate analysis of protein-protein interactions (PPIs) in living cells. We prepared stable cell lines that expressed polypeptides composed of an alpha h...

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Detalhes bibliográficos
Main Authors: Ting Chen, Ha Pham, Ali Mohamadi, Lawrence W. Miller
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020-09-01
Colecção:iScience
Assuntos:
Sensor
Molecular Spectroscopy Techniques
Molecular Interaction
Biomolecular Engineering
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2589004220307252
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