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Universal stem-loop primer method for screening and quantification of microRNA.
RT-qPCR is the accepted technique for the quantification of microRNA (miR) expression: however, stem-loop RT-PCR, the most frequently used method for quantification of miRs, is time- and reagent-consuming as well as inconvenient for scanning. We established a new method called 'universal stem-l...
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Auteurs principaux: | , , , , , , , , |
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Format: | Artigo |
Langue: | Inglês |
Publié: |
Public Library of Science (PLoS)
2014-01-01
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Collection: | PLoS ONE |
Accès en ligne: | http://europepmc.org/articles/PMC4280144?pdf=render |
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