Isolation of five different primary cell types from a single sample of human skin

Summary: We have developed a technique to isolate primary keratinocytes, melanocytes, fibroblasts, preadipocytes, and microvascular endothelial cells from an individual sample of human skin. The protocol describes step-by-step instructions for processing, cells isolation, and culture of neonatal for...

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Bibliographic Details
Main Authors: Sylwia Kabacik, Donna Lowe, Howard Cohen, Sarah Felton, Joseph Spitzer, Ken Raj
Format: Artigo
Language:Inglês
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Health Sciences
Clinical Protocol
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002581
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