Rapid Genome Sequencing of RNA Viruses

We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.

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Detalhes bibliográficos
Main Authors: Tetsuya Mizutani, Daiji Endoh, Michiko Okamoto, Kazuya Shirato, Hiroyuki Shimizu, Minetaro Arita, Shuetsu Fukushi, Masayuki Saijo, Chang Kweng Lim, Mikako Ito, Reiko Nerome, Tomohiko Takasaki, Koji Ishii, Tetsuro Suzuki, Ichiro Kurane, Shigeru Morikawa, Hidekazu Nishimura
Formato: Artigo
Idioma:Inglês
Publicado em: Centers for Disease Control and Prevention 2007-02-01
Colecção:Emerging Infectious Diseases
Assuntos:
Emerging infectious virus
SARS-CoV
West Nile virus
rapid determination system
whole genome amplification
direct sequencing
Acesso em linha:https://wwwnc.cdc.gov/eid/article/13/2/06-1032_article
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