Determination of neuraminidase kinetic constants using whole influenza virus preparations and correction for spectroscopic interference by a fluorogenic substrate.

The influenza neuraminidase (NA) enzyme cleaves terminal sialic acid residues from cellular receptors, a process required for the release of newly synthesized virions. A balance of NA activity with sialic acid binding affinity of hemagglutinin (HA) is important for optimal virus replication. NA sequ...

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Main Authors: Bindumadhav M Marathe, Vincent Lévêque, Klaus Klumpp, Robert G Webster, Elena A Govorkova
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science (PLoS) 2013-01-01
Serier:PLoS ONE
Online adgang:http://europepmc.org/articles/PMC3744557?pdf=render
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