Determination of neuraminidase kinetic constants using whole influenza virus preparations and correction for spectroscopic interference by a fluorogenic substrate.

The influenza neuraminidase (NA) enzyme cleaves terminal sialic acid residues from cellular receptors, a process required for the release of newly synthesized virions. A balance of NA activity with sialic acid binding affinity of hemagglutinin (HA) is important for optimal virus replication. NA sequ...

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Main Authors: Bindumadhav M Marathe, Vincent Lévêque, Klaus Klumpp, Robert G Webster, Elena A Govorkova
格式: Artigo
語言:Inglês
出版: Public Library of Science (PLoS) 2013-01-01
叢編:PLoS ONE
在線閱讀:http://europepmc.org/articles/PMC3744557?pdf=render
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