Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant <it>E. coli </it>strains with predesigned genome structure

<p>Abstract</p> <p>Background</p> <p>The development of modern producer strains with metabolically engineered pathways poses special problems that often require manipulating many genes and expressing them individually at different levels or under separate regulatory con...

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Bibliographic Details
Main Authors: Biryukova Irina V, Skorokhodova Aleksandra, Zimenkov Danila V, Gak Evgeny R, Minaeva Natalia I, Mashko Sergey V
Format: Artigo
Language:Inglês
Published: BMC 2008-08-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/8/63
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