Isolation of human intrahepatic leukocytes for phenotypic and functional characterization by flow cytometry

Summary: With the growing appreciation of tissue-resident immunity, studying tissue-specific immune cells contributing to both homeostasis and disease is imperative. Here, we provide a protocol for the isolation of human intrahepatic leukocytes (IHL) maximizing viability, purity, and yield. Our prot...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Stephanie Kucykowicz, Oliver E. Amin, Alice R. Burton, Leo Swadling, Nathalie M. Schmidt, Nekisa Zakeri, Jessica Davies, Gloryanne Aidoo-Micah, Kerstin A. Stegmann, Nicholas J. Easom, Anna Jeffery-Smith, Mala K. Maini, Laura J. Pallett
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2022-06-01
Colecção:STAR Protocols
Assuntos:
Cell isolation
Single Cell
Flow Cytometry/Mass Cytometry
Health Sciences
Immunology
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2666166722002362
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados