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A genetic assay for transcription errors reveals multilayer control of RNA polymerase II fidelity.
We developed a highly sensitive assay to detect transcription errors in vivo. The assay is based on suppression of a missense mutation in the active site tyrosine in the Cre recombinase. Because Cre acts as tetramer, background from translation errors are negligible. Functional Cre resulting from ra...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Public Library of Science (PLoS)
2014-09-01
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| Serier: | PLoS Genetics |
| Online adgang: | http://europepmc.org/articles/PMC4168980?pdf=render |
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