A genetic assay for transcription errors reveals multilayer control of RNA polymerase II fidelity.

We developed a highly sensitive assay to detect transcription errors in vivo. The assay is based on suppression of a missense mutation in the active site tyrosine in the Cre recombinase. Because Cre acts as tetramer, background from translation errors are negligible. Functional Cre resulting from ra...

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Huvudupphovsmän: Jordan D Irvin, Maria L Kireeva, Deanna R Gotte, Brenda K Shafer, Ingold Huang, Mikhail Kashlev, Jeffrey N Strathern
Materialtyp: Artigo
Språk:Inglês
Publicerad: Public Library of Science (PLoS) 2014-09-01
Serie:PLoS Genetics
Länkar:http://europepmc.org/articles/PMC4168980?pdf=render
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