A genetic assay for transcription errors reveals multilayer control of RNA polymerase II fidelity.

We developed a highly sensitive assay to detect transcription errors in vivo. The assay is based on suppression of a missense mutation in the active site tyrosine in the Cre recombinase. Because Cre acts as tetramer, background from translation errors are negligible. Functional Cre resulting from ra...

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Main Authors: Jordan D Irvin, Maria L Kireeva, Deanna R Gotte, Brenda K Shafer, Ingold Huang, Mikhail Kashlev, Jeffrey N Strathern
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science (PLoS) 2014-09-01
Serija:PLoS Genetics
Online dostop:http://europepmc.org/articles/PMC4168980?pdf=render
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