Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering

Abstract Background Medaka (Oryzias latipes) is a popular animal model used in vertebrate genetic analysis. Recently, an efficient (~ 30%) knock-in system via non-homologous end joining (NHEJ) was established in zebrafish using the CRISPR/Cas9 system. If the same technique were applicable in medaka,...

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Hlavní autoři: Ikuko Watakabe, Hisashi Hashimoto, Yukiko Kimura, Saori Yokoi, Kiyoshi Naruse, Shin-ichi Higashijima
Médium: Artigo
Jazyk:Inglês
Vydáno: BMC 2018-02-01
Edice:Zoological Letters
Témata:
Medaka
Transgenic
CRISPR/Cas9
Knock-in
On-line přístup:http://link.springer.com/article/10.1186/s40851-017-0086-3
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