High-Resolution 3D Heart Models of Cardiomyocyte Subpopulations in Cleared Murine Heart

Biological tissues are naturally three-dimensional (3D) opaque structures, which poses a major challenge for the deep imaging of spatial distribution and localization of specific cell types in organs in biomedical research. Here we present a 3D heart imaging reconstruction approach by combining an i...

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Detalhes bibliográficos
Main Authors: Huiying Ren, Zhaoli Pu, Tianyi Sun, Tangting Chen, Leiying Liu, Zhu Liu, Christopher O’Shea, Davor Pavlovic, Xiaoqiu Tan, Ming Lei
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2022-05-01
Colecção:Frontiers in Physiology
Assuntos:
HCN4 expression (HCN4+) pacemaker cells
Pnmt+ cell-derived cardiomyocytes (PdCMs)
heart tissue-clearing
light-sheet fluorescence microscopy
3D volume heart models
optogenetics
Acesso em linha:https://www.frontiersin.org/articles/10.3389/fphys.2022.779514/full
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